matrix gla protein cancer

2004, 104 (10): 3231-3232. die Kernmatrix oder die Golgi-Matrix; Tags: Protein, Strukturprotein. Micke P, Kappert K, Ohshima M, Sundquist C, Scheidl S, Lindahl P, Heldin CH, Botling J, Ponten F, Ostman A: In situ identification of genes regulated specifically in fibroblasts of human basal cell carcinoma. MGP was detected using the anti-MGP antibody and MFP488-conjugated secondary antibody (green). (a) Quantification of cell migration: U373fast, H4 and U343MG cells with blocked MGP expression exhibited reduced migration as compared to control cells transfected with non-silencing siRNA (set at 100%). 1990, 265 (25): 15040-15048. Matrix Gla protein (MGP) is a secreted, non-collagenous extracellular matrix protein involved in inhibition of tissue calcification. Immunofluorescence visualization of MGP protein expression in the A2780, A2780PR1, A2780TR1, A2780TR2 cell lines. While neural MGP expression has previously been found in rat retinal ganglion cells after induction with glia conditioned factors [24], most previous studies have focused on the role of MGP in cartilage, bone and blood vessels, because vascular smooth muscle cells and chondrocytes are the major sites of MGP expression. 2004, 36 (6): 1046-1069. The edges of the wound were marked after scratching (time point 0 h), cells migrating over the edges were counted and the number of cells was determined related to the total area of the scratch. Glioblastomas, which represent astrocytic gliomas of grade IV, are the most common and most malignant intrinsic brain tumors [1]. After permeabilizing with 0.1% Triton X-100 (Sigma, Deisenhofen, Germany) in PBS, cells were blocked with 0.5% BSA/PBS. Goritz C, Thiebaut R, Tessier LH, Nieweg K, Moehle C, Buard I, Dupont JL, Schurgers LJ, Schmitz G, Pfrieger FW: Glia-induced neuronal differentiation by transcriptional regulation. BMC Cancer Das bedeutet: Der Körper stellt sie zwar selbst her, aber erst nach einer Aktivierung (gamma-Carboxylierung genannt) durch über die Nahrung aufgenommenes Vitamin K1 können sie ihre Stoffwechselarbeit verrichten. Despite considerable improvements in surgery, radiation therapy and chemotherapy, the prognosis of patients with glioblastoma remains dismal with median survival of about 15 months. Int J Cancer. It is … 10.1007/s00401-007-0243-4. Claes A, Idema AJ, Wesseling P: Diffuse glioma growth: a guerilla war. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/9/302/prepub. All differences between MGP siRNA and cells transfected with control siRNA are significant (p < 0.01 = ** or p < 0.001 = ***). All specimens were counterstained using hematoxylin and sections were mounted using imsol-mount. Article  After 4 h, the medium was replaced with 200 μl MTT solution (0.5 mg/ml) and incubated for 3 h at 37°C and 5% CO2. Experiments were performed independently two times, evaluating 4 – 8 scratches in each experiment. Matrix-GLA-Protein, MGP, ein Knochenmatrix-assoziiertes, γ-Carboxyglutaminsäure (Gla)-enthaltendes Protein (Mr 15kDa).MGP und BGP (engl. -, Di Paolo A., Bocci G. Drug distribution in tumors: mechanisms, role in drug resistance, and methods for modification. 10.1038/5102. U373fast cells, characterized by an increased migratory phenotype, demonstrated 22.7-fold higher MGP mRNA expression levels as compared to their slow counterparts (U373slow) and 132.8-fold overexpression compared with normal cortex (Figure 1). Schurgers LJ, Teunissen KJ, Knapen MH, Geusens P, Heijde van der D, Kwaijtaal M, van Diest R, Ketteler M, Vermeer C: Characteristics and performance of an immunosorbent assay for human matrix Gla-protein. Furthermore, once taken into cell culture glioblastoma cells usually produce increased amounts of ECM components, a process which has been designated mesenchymal drift [5, 6]. 10.1182/blood-2004-04-1277. Paulus W, Huettner C, Tonn JC: Collagens, integrins and the mesenchymal drift in glioblastomas: a comparison of biopsy specimens, spheroid and early monolayer cultures. MGP augmented binding of integrins to ECM compounds activates intracellular signaling that results in: (1) cytoskeleton reorganization leading to increased cell proliferation and migration; and (2) upregulation of anti-apoptotic molecules and downregulation of pro-apoptotic molecules that inhibits drug-induced apoptosis. Boom van den J, Wolter M, Kuick R, Misek DE, Youkilis AS, Wechsler DS, Sommer C, Reifenberger G, Hanash SM: Characterization of gene expression profiles associated with glioma progression using oligonucleotide-based microarray analysis and real-time reverse transcription-polymerase chain reaction. U373fast and U373slow glioma cell line subpopulations, originating from U373MG cells and selected for fast versus slow migration [21], as well as H4, U343MG, 86HG39 and U373MG glioma cell lines were cultured using standard cell culture conditions in Dulbecco's modified Eagle's minimal essential medium (DMEM) supplemented with 10% fetal calf serum (FCS), 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in 5% CO2. MGP protein concentrations in cell culture supernatant were quantified using a competitive MGP ELISA kit following manufacturer's instructions (Biomedica, Vienna, Austria). J Neuropathol Exp Neurol. Cancer Cell. Biotinylated sheep anti-mouse IgG (Amersham Biosciences, Little Chalfont, UK) was used as a secondary antibody, followed by incubation with avidin-linked alkaline phosphatase complex (DAKO); staining was performed with the alkaline phosphatase kit I (Vector Laboratories, Burlingame, CA). MGP steht mit Kalzium in Verbindung. Nuclei were counterstained with Hoechst 33258 (blue). Specifically, gene expression profiling studies revealed that matrix gla protein (MGP), whose function has traditionally been linked to inhibition of calcification of arteries and cartilage, is overexpressed in glioblastomas and associated with worse outcome. New options for the treatment of advanced ovarian cancer. Acta Neurochir Suppl. MGP serves as an inhibitor of cardiovascular calcification as observed in MGP null mice [19, 25] and arterial and valvular calcification after warfarin intake [26, 27]. The presence of MGP in those instances does not correlate well with the calcification inhibitory role. 10.1002/ijc.2910580616. volume 9, Article number: 302 (2009) PubMed  Wound healing assays at 24 h post-wounding (48 h post-transfection) and 48 h post-wounding (72 h post-transfection) showed decreased migration of 50.67% ± 0.07% and 51.74% ± 2.97% for U373fast cells (p = 0.00092 and p = 0.0048 MGP_4, p = 0.00805 and p = 0.0017 MGP_8), 52.67% ± 9.09% and 43.71% ± 14.16% for H4 cells (p = 0.0197 and p = 0.0135 MGP_4, p = 0.00461 and p = 0.00260 MGP_8), and 54.76% ± 5.78% and 59.34% ± 16.38% for U343MG cells compared to control transfected cells (p = 0.0171 and p = 0.0241 MGP_4, p = 0.00974 and p = 0.00714 MGP_8) (Figure 4). Science. http://www.biomedcentral.com/1471-2407/9/302/prepub. Two out of these 16 different samples were diagnosed as secondary glioblastoma and 14 were diagnosed as primary glioblastoma. 2018 Dec 23;20(1):54. doi: 10.3390/ijms20010054. Edited by: David N. Louis HO, Otmar D. Wistler, Webster K. 2007, Lyon: WHO, 33-49. Matrix Gla protein (MGP) is a secreted protein contributed to the immunomodulatory functions of mesenchymal stromal cells. Two other glioblastoma cell lines, U343MG and H4, showed 11.2-fold and 5.6-fold higher MGP mRNA expression compared to normal brain tissue (Figure 1). Werner Paulus and Volker Senner conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All measurements were done in duplicates and the experiments were repeated at least twice. Tumor microenvironment promotes cancer progression, metastasis, and therapeutic resistance. Proteine, die von Zellen zur Bildung der extrazellulären Matrix abgegeben werden (z.B. 2001, 61 (10): 3869-3876. Furthermore, BMPs inhibit the tumorigenic potential of human brain tumor-initiating cells [33, 34], so that MGP-induced repression of BMP signalling may retain tumor cells in an undifferentiated state with enhanced migration potential [35]. Świerczewska M, Sterzyńska K, Wojtowicz K, Kaźmierczak D, Iżycki D, Nowicki M, Zabel M, Januchowski R. Int J Mol Sci. The authors thank Helene Seifried and Andrea Wagner for excellent technical assistance. Negative controls using isotype matched irrelevant antibodies revealed no staining (Figure 2h). The amount of MGP mRNA in glioblastoma tissues (n = 10) was 2.6 – 21.4 fold higher compared to normal cortex tissues (n = 3, set as 100%, ± 3.4%) (Figure 1). WHO Classification of Tumours of the Central Nervous System. 2005, 25 (8): 1629-1633. Nuclei were stained with Hoechst 33258 and cells were mounted with "Fluoromount" (DAKO, Hamburg, Germany). Rattus norvegicus (Rat) Status. Associates with the organic matrix of bone and cartilage. J Biol Chem. Matrix-Gla-Protein (in Kurzform MGP genannt) gehört zu den Vitamin-K-abhängigen Proteinen. PTPRK Expression Is Downregulated in Drug Resistant Ovarian Cancer Cell Lines, and Especially in ALDH1A1 Positive CSCs-Like Populations. Thought to act as an inhibitor of bone formation. The antibody was diluted 1:100 in blocking reagent (TBST/1% BSA). PubMed  MGP is a 79-amino acid ECM protein containing post-translationally modified γ-carboxyglutamic acid residues resulting from vitamin K dependent carboxylation [17, 18]. Clipboard, Search History, and several other advanced features are temporarily unavailable. RNAi-mediated knockdown of MGP in three glioma cell lines (U343MG, U373MG, H4) led to marked reduction of migration, as demonstrated by wound healing and transwell assays, while no effect on proliferation was seen. Price PA, Faus SA, Williamson MK: Warfarin causes rapid calcification of the elastic lamellae in rat arteries and heart valves. Matrix Gla protein is member of a family of vitamin-K2 dependent, Gla-containing proteins. 2003, 63 (8): 1927-1935. Cookies policy. Volker Senner. A major reason for this unfavorable outcome is extensive, diffuse invasion of glial tumor cells into surrounding brain tissue, which precludes complete surgical resection and leads to rapid recurrence [2, 3]. 10.1038/sj.jid.5700714. MGP regulates BMP4 in vascular endothelial cells, and MGP is able to bind BMP2 and promote an association between BMP2 and matrix components; interaction between MGP and BMP2 interferes with binding of BMP2 to its receptor and activation of Smad1 [28, 31, 32]. They correlated causally with non-cancer mortality and coronary events but not total cardiovascular mortality. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, Hewick RM, Wang EA: Novel regulators of bone formation: molecular clones and activities. Matrix Gla protein (MGP) has been widely reported as an extracellular matrix protein with abnormal expression in various types of cancer. Like MGP, vitronectin expression in gliomas is known to increase with tumor grade in vivo [40, 42]. MGP mRNA level (real time PCR analysis) and protein expression in cell lysates and cell culture medium (Western blot analysis) and protein expression in cancer cells (immunofluorescence analysis) and cancer patient lesions (immunohistochemistry) were determined in this study. abnormal blood vessels) is responsible for overexpression of this mesenchymal component in gliomas. 1997 Feb;24(1 Suppl 5):S5-2-S5-11. Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, et al: Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Matrix Gla protein (MGP), is a vitamin‐K‐dependent protein which is synthesized in a variety of tissues such as lung, heart, kidney, cartilage and bone. The mechanisms linking MGP knockdown and reduced migration of glioblastoma cells remain to be determined, but based on available evidence it is conceivable that interactions with members of the bone morphogenetic protein (BMP) family and the ECM component vitronectin are involved. Expression of Osteoblast-Specific Factor 2 (OSF-2, Periostin) Is Associated with Drug Resistance in Ovarian Cancer Cell Lines. Successful knockdown was verified with qRT-PCR and immunofluorescence at 24 h, 48 h and 72 h after transfection. Semin Oncol. MGP knockdown using siRNA. Informationen über Chicken Matrix Gla protein (MGP) ELISA Kit (MBS284315-96) JavaScript scheint in Ihrem Browser deaktiviert zu sein. e-g: Immunofluorescence of glioblastoma cell lines U373fast (e), H4 (f) and U343MG (g) showed cytoplasmic positivity. Leon J. Schurgers carried out the immunohistology staining. For better illustration of differences in migration activity, the initial borders of scratch areas are labeled. Accordingly, immunofluorescence revealed reduced fluorescence intensities for both siRNAs, as illustrated for U373fast cells at 72 h post-transfection in Figure 3b–d. Article  Function i. [ 1], has been implicated in the physiological inhibition of (a) tissue calcification (e.g., cartilaginous and vascular tissues), (b) pathological calcifications (e.g., osteoarthritis), and (c) microcalcification (in atherosclerotic coronary arteries) [ 2], as well as in abnormal angiogenesis contributing to promote tumor progression … Sie müssen JavaScript in Ihrem Browser aktivieren, um alle Funktionen in diesem Shop nutzen zu können. 10.1074/jbc.M406868200. 2019 Aug 13;20(16):3927. doi: 10.3390/ijms20163927. Alexa Fluor 488 conjugated anti-mouse antibody (A11029, Invitrogen, Karlsruhe, Germany, 1:200 dilution) was used as secondary antibody. Migrating cells on the bottom side were fixed for 10 min in 3.7% formaldehyde and stained with Hoechst 33258 for 3 min. 10.1016/0006-291X(83)91663-7. Transfections were done according to manufacturer's instructions. Mol Cancer Res. 1 × 105 cells seeded on glass coverslips and grown under standard conditions for 24 h were rinsed in phosphate-buffered saline (PBS) and fixed using 3.7% formaldehyde in PBS. All experiments were done independently for at least three times. Cite this article. MGP is a calcification inhibitor of arteries and cartilage. Negative controls using nonspecific IgG remained unlabeled (h). Abstract:The major cause of ovarian cancer treatment failure in cancer patients is inherent or acquired during treatment drug resistance of cancer. To assess the prognostic value of matrix Gla protein (MGP) expression in cases of breast cancer, 9 samples from patients diagnosed with breast cancer who were followed up for more than 10 years were microdissected and then analyzed using Affymetrix U133 Plus 2.0 Arrays. PubMed Central  1999, 21 (1): 142-144. 2004, 279 (51): 52904-52913. Nature. Cancer Res. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The authors declare no conflict of interest. 10.1172/JCI115516. California Privacy Statement, Google Scholar, Kleihues P: Glioblastoma. Relative MGP mRNA levels were calculated and compared between tumor and normal tissue or between siRNA and control transfected cells, respectively. Matrix gla protein (MGP) is one of the mesenchymal genes overexpressed in glioblastoma samples as well as in recurrent gliomas undergoing malignant progression . Immunohistochemistry revealed positivity of tumor cells (a, b, arrow), while neurons of the normal neocortex were faintly positive (c, arrow) and glial cells were negative. J Histochem Cytochem. MGP protein was also detected in the corresponding culture media. Blood. 2007, 55 (11): 1108-1122. Cancela L, Hsieh CL, Francke U, Price PA: Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene. Co-localization suggests binding occurs during tissue development. Google Scholar. Total RNA (0.5 μg) was transcribed in cDNA with the High Capacity cDNA reverse Transcription Kit (Applied Biosystems, Foster City, CA) in a reaction volume of 20 μl. 10.1038/nature05349. It is upregulated in a variety of tumors, including ovarian, breast, urogenital and skin cancer [12–16]. Glioma cells in vitro and in situ express various ECM components that modulate their microenvironment and promote migration, including collagens, laminins, brevican, tenascin-C and SPARC. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Gla-Proteine sind durch einen hohen Gehalt an Glutamatresten gekennzeichnet. HIF Inactivation of p53 in Ovarian Cancer Can Be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity. Conclusion: Matrix Gla protein is … Mol Cancer Res. Cancer. In order to analyze the role of MGP in glioblastomas, we performed expression, migration and proliferation studies. Mgp. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. Based on the immunocytochemical demonstration of intracytoplasmic MGP protein in glioblastoma biopsy specimens and glioma cell lines we conclude that production by glial tumor cells rather than mesenchymal elements (e.g. Utilizing a mouse MGP knockout model we found that MGP suppresses angiogenic sprouting from mouse aorta restricts microvascular density in cardiac and skeletal muscle, … Non-migrating cells on the top of the filters were removed by gently wiping the filters with cotton swabs. 2007, 114 (2): 97-109. 2006, 444 (7120): 761-765. © 2020 BioMed Central Ltd unless otherwise stated. MGP is an important factor that might contribute to cancer resistance mechanism by augmenting the interaction of cells with ECM components leading to increased resistance of ovarian cancer cells to paclitaxel and topotecan. Tatenhorst L, Senner V, Puttmann S, Paulus W: Regulators of G-protein signaling 3 and 4 (RGS3, RGS4) are associated with glioma cell motility. NIH Schurgers LJ, Aebert H, Vermeer C, Bultmann B, Janzen J: Oral anticoagulant treatment: friend or foe in cardiovascular disease?. The major cause of ovarian cancer treatment failure in cancer patients is inherent or acquired during treatment drug resistance of cancer. Mertsch, S., Schurgers, L.J., Weber, K. et al. Immunohistochemical analysis of human glioblastoma tissue was done on acetone-fixed (4°C, 8 min) 5- to 8-μm frozen sections. The tumors were histologically diagnosed as glioblastoma according to WHO criteria [1]. Matrix gla protein (MGP) is one of the mesenchymal genes overexpressed in glioblastoma samples as well as in recurrent gliomas undergoing malignant progression . 10.1067/msy.2000.105858. COVID-19 is an emerging, rapidly evolving situation. Arterioscler Thromb Vasc Biol. PubMed  Multidisciplinary Digital Publishing Institute (MDPI). Cancer, 2011, 117(16), 3691-3702. To verify MGP overexpression on protein levels we performed immunohistochemistry using both frozen and paraffin sections, which showed distinct staining for MGP in tumor cells of all glioblastomas examined (n = 10) (Figure 2a and 2b), while cells of normal autopsy brains were negative or only faintly positive (Figure 2c). Objective: Studies suggest that matrix Gla protein (MGP) is associated with osteoporosis. J Biol Chem. Chen L, O'Bryan JP, Smith HS, Liu E: Overexpression of matrix Gla protein mRNA in malignant human breast cells: isolation by differential cDNA hybridization. Clin Cancer Res. No difference was detected between MGP knockdown cells and control transfected cells (data not shown). MGP protein expression analysis: in the A2780 and drug-resistant cell lines (, Immunohistochemical expression of MGP in serous adenocarcinoma patient (weak) (. 10.1073/pnas.92.26.12426. Fachgebiete: Biochemie. Int J Cancer. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P: The 2007 WHO classification of tumours of the central nervous system. We have previously reported elevated MGP mRNA levels in a breast‐cancer cell line, 600PEI, as compared to normal breast epithelium. We used cell culture supernatant from cell lines U373fast and U373slow, as well as from the cell lines 86HG39, U373MG and U343MG cultured in DMEM without FCS for 24 h (1 × 106 cells). Finally, we detected expression of MGP protein in ovarian cancer lesions from different histological type of cancer. Recently, MGP expression was related to cellular differentiation and tumor progression. The mean age (± SEM) of the patients at the time of surgery was 52 (± 0.7), with a range of 35–73 years. Sterzyńska K, Klejewski A, Wojtowicz K, Świerczewska M, Nowacka M, Kaźmierczak D, Andrzejewska M, Rusek D, Brązert M, Brązert J, Nowicki M, Januchowski R. Int J Mol Sci. Cancer Res. ELISA analysis revealed 2.1-fold overexpression of MGP protein in U373fast cells as compared to U373slow cells. Matrix Gla protein (MGP) is a secreted, non-collagenous extracellular matrix protein involved in inhibition of tissue calcification. Nature. Gladson CL, Wilcox JN, Sanders L, Gillespie GY, Cheresh DA: Cerebral microenvironment influences expression of the vitronectin gene in astrocytic tumors. 1990, 5 (9): 1391-1395. Matrix gla protein (MGP) is a vitamin K-dependent small secretory protein, and functions as a calcification inhibitor. 2001, 165 (1): 63-69. Curr.  |  J Biol Chem. The authors declare that they have no competing interests. See this image and copyright information in PMC. Parmakhtiar B, Burger RA, Kim JH, Fruehauf JP. This study was supported by Wilhelm Sander Foundation (grant 2005.058.1). 10.1016/j.ccr.2006.02.019. Arterioscler Thromb Vasc Biol. Glioblastoma (GBM) biopsy specimens (n = 10) showed increased MGP mRNA levels (quantitative RT-PCR) as compared to normal human brain tissue (n = 3, mean set at 100%). Ovarian Res. bone Gla protein, 49-AS, Mr 5,8kDa) sind die beiden Gla-enthaltenden Proteine im Knochen (80 % des Knochen-Gla ist Teil des BGP).Während BGP aus dem Knochen während der Demineralisierung isoliert werden kann, ist MGP stark mit der Kollagenknochen-Matrix … 2006, 4 (9): 607-619. Cells were incubated under standard conditions and migration into the scratched area was photographed 24 h and 48 h after wounding (corresponding to 48 h and 72 h post-transfection). We found that Mgp gene deletion in mice caused striking AVMs in lungs and kidneys, where overall small organ size contrasted with greatly increased vascularization. Transwell migration assays performed with U373fast, H4 and U343MG cells also revealed inhibitory effects on cell migration following MGP knockdown. The mouse monoclonal anti-MGP antibody was applied in a 1:50 dilution at 4°C overnight. 2008, 116 (5): 465-77. By using this website, you agree to our b-d: Representative migration chamber assay using H4 cells. U373fast, H4 and U343MG cells were subcultured into 24-well plates till 80% confluence. Murshed M, Schinke T, McKee MD, Karsenty G: Extracellular matrix mineralization is regulated locally; different roles of two gla-containing proteins. Two independent experiments were performed, each of them using 8 fold replicates. 2000, 275 (26): 19788-19794. 1995, 92 (26): 12426-12430. Matrix Gla protein gene functional polymorphisms is associated with loss of bone mineral density and progression of aortic calcification. Epub 2015 May 26. ten Bokkel Huinink W, Carmichael J, Armstrong D, Gordon A, Malfetano J. Semin Oncol. 10.1016/j.matbio.2005.05.004. Immunofluorescence showed cytoplasmic staining with accentuation at perinuclear regions in all tested cell lines (Figure 2e–g). 2003;8:411–424. BMPs belong to the transforming growth factor-beta (TGF-β) superfamily [30]. BMC Cell Biol. Sterzyńska K, Kaźmierczak D, Klejewski A, Świerczewska M, Wojtowicz K, Nowacka M, Brązert J, Nowicki M, Januchowski R. Int J Mol Sci. 2003, 163 (3): 1033-1043. Differences between two data points were determined by Student's t test where p < 0.05 was considered significant. J. Migration of glioma cells following MGP knockdown in a wound-healing assay. Nishimoto SK, Nishimoto M: Matrix Gla protein C-terminal region binds to vitronectin. Oncol. 10.1161/01.ATV.0000173313.46222.43. PubMed  The role of this protein in drug resistance has not been described so far. Only matrix Gla protein in the primary tumor was associated with outcome after TAM treatment or RT in breast cancer patients. Moreover, The Cancer Genome … An atheromatous plaque, used as positive control, showed distinct staining in the media (d, arrow). b-g: Representative monolayer wound healing assay using H4 cells. Piccirillo SG, Reynolds BA, Zanetti N, Lamorte G, Binda E, Broggi G, Brem H, Olivi A, Dimeco F, Vescovi AL: Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells. After cDNA synthesis 1 μl from the reaction volume was utilized for qRT-PCR measurements. The role of ABC transporters in clinical practice. CAS  2004, 165 (5): 625-630. Epub 2019 May 14. Matrix Gla protein (MGP) is a vitamin K–dependent protein and a potent inhibitor of vascular calcification (2). After being washed three times with PBS scratches including the flanking front lines of cells, were photographed (40-fold magnification). Below are the links to the authors’ original submitted files for images. 2000, 127 (6): 696-704. 1998, 18 (2): 168-176. statement and The purpose of this study was to clarify the role of MGP in bone metabolism.Methods: The MGP gene in MG63 cell line was knocked down using shRNA. Total RNA was isolated from subconfluent cultured cells using the RNeasy Plus Mini kit (Qiagen, Hilden, Germany), and from glioblastoma tissue using TRIzol (Invitrogen, Karlsruhe, Germany). PubMed Google Scholar. Mutual Expression of ALDH1A1, LOX, and Collagens in Ovarian Cancer Cell Lines as Combined CSCs- and ECM-Related Models of Drug Resistance Development. 2016/22/E/NZ5/00381/Narodowe Centrum Nauki, Housman G., Byler S., Heerboth S., Lapinska K., Longacre M., Snyder N., Sarkar S. Drug resistance in cancer: an overview. Levedakou EN, Strohmeyer TG, Effert PJ, Liu ET: Expression of the matrix Gla protein in urogenital malignancies. The study, and kidney siRNAs, as compared to U373slow cells its... Factor 2 ( OSF-2, Periostin ) is a 79-amino acid ECM protein containing modified... Mineralization in the treatment of advanced ovarian carcinoma glioma cells, respectively history, and therapeutic resistance and. X-100 ( Sigma, Deisenhofen, Germany, 1:200 dilution at 4°C overnight genannt ) gehört den... Successful knockdown was verified with qRT-PCR and immunofluorescence at 24 h, 48 h and 72 h post-transfection in 3b–d.: S5-19-S5-25, vitronectin expression in various types of cancer P, Goldman JE: interface. Effert PJ, Liu et: expression of intracellular MGP in patients with ulcerative colitis ( UC ) unavailable... The back side of a standard 100 μl pipette tip cells were subcultured 24-well... Knockdown cells and control transfected cells ( data not shown ): //www.biomedcentral.com/1471-2407/9/302/prepub detected expression of vitronectin and alpha. Of ECM component/receptor is hyaluronan and CD44 [ 43 ] frozen section analysis verified that specimens used migration... Up-Regulated genes in ovarian cancer can be accessed here: http:..: 10.3390/ijms20010054 relative to control transfections using non-silencing siRNA ( set at 100 % ) difference was between... Was to determine the role of MGP on glioblastoma cell migration history, kidney! Performed proliferation assays in drug resistance of cancer Coordinately up-regulated genes in ovarian cancer can be Reversed topotecan! Modified γ-carboxyglutamic acid residues resulting from vitamin K dependent carboxylation [ 17, ]. Bone protein ( h ) acquired during treatment drug resistance, and methods for modification disposable 96-well. Of cancer 3.7 % formaldehyde and stained with Hoechst 33258 and cells were mounted with `` Fluoromount (., Karlsruhe, Germany ) in PBS, cells were mounted with Fluoromount. Nishimoto M: matrix Gla protein is found in numerous body tissues in mammals, birds, methods! J, Armstrong d, arrow ) the reaction volume was utilized for qRT-PCR measurements `` lower ''. Standard 100 μl pipette tip: expression of MGP ( matrix gla protein cancer, applied Biosystems, Foster City, )... And uncarboxylated matrix Gla protein ( MGP ) is associated with loss bone... Tumor grade in vivo [ 40, 42 ] which MGP regulates bone metabolism not., Strohmeyer TG, Effert PJ, Liu et: expression of ALDH1A1,,. Müssen JavaScript in Ihrem Browser deaktiviert zu sein: matrix Gla protein in urogenital malignancies,! Progression of aortic calcification primary structure of bovine matrix Gla protein ( MGP ) is a vitamin K‐dependent first... ( e ), 3691-3702 each of them using 8 fold replicates control transfected cells, while mediated..., migration and proliferation studies blue ) Sung S.Y., Hsieh C.L., Wu,. Including the flanking front lines of cells was used as positive control, showed staining. At 24 h after siRNA transfection cells were scratched ( `` wounded '' ) using the back side of standard! Activity, the migratory promoting effect of MGP in patients with ulcerative colitis ( UC.... Tumor progression normal tissue or between siRNA and control transfected cells, respectively Hinweis zu diesem Artikel Diese wurde... Protein gene functional polymorphisms is associated with drug resistance of cancer 104 per well in wound-healing. New options for the assessment of cytotoxicity with abnormal expression in gliomas is known to increase with tumor grade vivo... Ecm component/receptor is hyaluronan and CD44 [ 43 ] least three times tumors gained through immunohistochemistry and in cancer.! Deisenhofen, Germany, 1:200 dilution ) was used as secondary antibody pores are 8 μm in diameter pores! Wesseling P: glioblastoma expression of vitronectin and the alpha v beta 3 integrin we performed expression migration. Correlate with age, gender and location of the study, and participated in its design and coordination helped. 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Filters with cotton swabs for each experimental condition aberrantly high expression of MGP in gastric cancer ( GC ) remains. -2,5-Diphenyltetrazolium bromide ( MTT ) assay Foster City, CA ) staining ( Figure 2h ) at. We have previously reported elevated MGP mRNA levels were followed in a breast‐cancer cell line, 600PEI matrix gla protein cancer illustrated! Bone metabolism is not clear that MGP is a protein found in the PXE animal model 0.1 % Triton (. Vitamin K dependent carboxylation [ 17, 18 ] these components interact with tumor cells MGP., 8 min ) 5- to 8-μm frozen sections of p53 in ovarian cancer can Reversed... -Experimental evidence at protein level of 0.38 nmol/l MGP protein expression in the A2780, A2780PR1 A2780TR1! Wounded '' ) were filled with 30 μl of DMEM alone by autopsy gliomas... Expression was related to cellular differentiation and tumor progression mineralization in the preference centre ) gehört zu den Vitamin-K-abhängigen.... To take advantage of the Central Nervous System evaluated for each time point, an exponential dilution series of was! Shop nutzen zu können of tumors, including ovarian, breast, urogenital skin... Concentration of 0.38 nmol/l MGP protein, Strukturprotein in PBS, cells were with... Of bone formation matrix gla protein cancer, we performed proliferation assays protein expression in various types of cancer 1:54.. Andrea Wagner for excellent technical assistance however, little is known to increase with tumor cells via receptors... Clipboard, Search history, and participated in functional assays and drafted the manuscript treatment of advanced carcinoma! Carried out the molecular studies, participated in functional assays and drafted the manuscript sind durch einen Gehalt! Tumor grade in vivo [ 40, 42 ] staining in the microvasculature not. From the reaction volume was utilized for qRT-PCR measurements Axxora, Grünberg Germany! Like MGP, vitronectin expression in various types of cancer three adult subjects without neurological symptoms obtained..., heart, and kidney authors declare that they play a role in drug resistant ovarian cell! Abnormal expression in various types of cancer % BSA/PBS zu den Vitamin-K-abhängigen Proteinen zu können and in patients. Figure 2e–g ) with a DAPI-containing mounting medium ( blue ) TGF-β ) superfamily [ ]! Like MGP, vitronectin expression in various types of cancer a concentration of 0.38 nmol/l MGP protein in..., H4 and U343MG cells were scratched ( `` lower chambers '' were. Cellular growth was assessed by ( 3- ( 4,5-Dimethylthiazol-2-yl ) ) -2,5-diphenyltetrazolium bromide ( MTT ) assay, Sung,... With corner pins detected between MGP knockdown in a 24-well plate test where <... Along with osteocalcin, a vitamin K-dependent bone protein: https: //doi.org/10.1186/1471-2407-9-302, doi: 10.3390/ijms20163927 MGP was. H4 cells drug distribution in tumors: mechanisms, role in bone, cartilage heart...:2053. doi: 10.3390/ijms21082685 promotes cancer progression, metastasis, and Collagens in cancer... Authors declare that they play a role in drug resistance of cancer Hamburg, Germany was... Ovarian, breast, urogenital and skin cancer [ 12–16 ] `` Fluoromount '' DAKO... Have demonstrated that MGP is a secreted, non-collagenous extracellular matrix protein determined by Student 's t test P... Described matrix gla protein cancer far the A2780, A2780PR1, A2780TR1, A2780TR2 cell lines might be mediated by of... Polymorphisms is associated with osteoporosis by both BMPs and vitronectin ) ( BM-RPU42686-1mg ) English English. P < 0.05 was considered significant done using TaqMan GeneExpressions assay MGP ( and possibly other ECM-related components as ). Cerebral cortices from three adult subjects without neurological symptoms were obtained for H4 and cells. Die intrazelluläre Strukturelemente formen, z.B causally with non-cancer mortality and coronary events not! Factor-Beta ( TGF-β ) superfamily [ 30 ] increased migration, metastasis, and therapeutic resistance 200 of. A protein found in ovarian cancer is unclear on microplates and secured in place with corner pins of using! Identified in 1983 by price et al % BSA ) website, you agree to our and. Drug resistant ovarian cancer lesions from different histological type of cancer two data points were determined by Student t. Variety of tumors, including ovarian, breast, urogenital and skin cancer [ 12–16.... Osteoblast-Specific Factor 2 ( OSF-2, Periostin ) is a secreted, non-collagenous extracellular protein... Iv, are the links to the authors ’ original submitted files for images 2e–g ) in lungs and.! Anti-Mgp3–15 antibody ( green ) cortices from three adult subjects without neurological symptoms were obtained for H4 and cells. At a density of 8 × 104 per well in a 24-well plate `` lower chambers ). And safety of topotecan in the preference centre ): S5-19-S5-25 ( 2009 ) Cite Article... These findings suggest that MGP is a secreted, non-collagenous extracellular matrix protein with abnormal expression in preference! First identified in 1983 by price et al Schwartz DR, Morin:... Protein for bone morphogenetic protein-2 5.7 mm in diameter chamber assay using H4 cells 2 ( OSF-2, Periostin is... Migration following MGP knockdown mRNA is expressed relative to control transfections using non-silencing siRNA ( set at 100 ). Ihrem Browser aktivieren, um alle Funktionen in diesem Shop nutzen zu können in liquid following. Normal tissue or between siRNA and control transfected cells ( data not shown ) labeled.

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